Cardiogen

A synthetic tetrapeptide (Ala-Glu-Asp-Arg) studied in preclinical models of myocardial tissue culture, peptide–chromatin interaction biochemistry, and gene-expression regulation.

Molecular Profile

Type
Synthetic linear tetrapeptide (short peptide bioregulator)
Molecular formula
C18H31N7O9
Molecular weight
489.5 g/mol
Amino acids
4
Sequence
Ala-Glu-Asp-Arg (AEDR); systematic form H-Ala-Glu-Asp-Arg-OH
Modification
None (unmodified free peptide: free N-terminus, free C-terminus)

Mechanism & Target Class

Cardiogen belongs to the class of ultra-short regulatory peptides (2–4 residues) termed peptide bioregulators. Its sequence contains acidic residues (Glu, Asp) and a basic residue (Arg), giving it a charged, amphiphilic character proposed to support interaction with nucleic acids and nuclear proteins. The mechanistic hypothesis examined for this peptide class is that short peptides can enter cells and the nucleus without a classical carrier and interact site-specifically with DNA — including methylated CNG/CG-containing sequences — and with N-terminal regions of histones, thereby influencing the accessibility of gene-promoter regions and the activity of DNA-processing enzymes. In structural and biophysical work, short peptides of this class have been modeled as binding the minor groove of double-stranded DNA. These proposals are derived from in vitro and in silico systems; the precise promoter sequences engaged by AEDR in cardiac cells have not been fully characterized in the published literature.

Storage & Handling

Lyophilized
Store sealed and protected from light, frozen (−20 °C, or colder for long-term storage), and desiccated.
Handling
As a small unmodified linear peptide with charged residues, susceptible to proteolysis and degradation from heat, light, and repeated freeze–thaw. Handle using standard aseptic technique. For research use only.

Primary Database

PubChem CID 11583989 ↗

References (22)

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