B7-33
A synthetic 27-residue single-chain peptide based on the relaxin B-chain, studied in receptor-pharmacology and structural research.
Molecular Profile
- Type
- Peptide (single-chain relaxin analog)
- Molecular formula
- C131H229N41O36S
- Molecular weight
- 2986.58 Da
- CAS number
- 1818415-56-3
- Amino acids
- 27
- Sequence
- Val-Ile-Lys-Leu-Ser-Gly-Arg-Glu-Leu-Val-Arg-Ala-Gln-Ile-Ala-Ile-Ser-Gly-Met-Ser-Thr-Trp-Ser-Lys-Arg-Ser-Leu
- Modification
- C-terminal amide; N-terminus free; no disulfide bonds (unlike the native two-chain relaxin hormone).
Mechanism & Target Class
B7-33 is a linear mimetic of the relaxin B-chain that engages a class B1 LGR-family G protein-coupled receptor, contacting its leucine-rich-repeat extracellular domain in a manner analogous to natural relaxin but lacking the disulfide-linked A-chain, producing a compact single-chain format. Structural studies characterize this single-chain binding mode relative to the native two-chain hormone, and its signaling profile in cell-based assays is described as distinct from that of the full relaxin hormone.
Storage & Handling
- Lyophilized
- Store at –20 °C long term; brief refrigeration (2–8 °C) is acceptable. Protect from moisture and light.
- Handling
- Aliquot to avoid repeated freeze-thaw cycles; peptide may degrade in aqueous solution. Avoid bacterial contamination.
Primary Database
References (22)
Reviews
1Wołowiec ŁW, Jaśniak A, Osiak-Gwiazdowska J, Czaplińska D, Szymczak A, Pęcherz JA, Grześk G. (2025). Frontiers in Pharmacology
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Bathgate RAD, Kocan M, Scott DJ, Hossain MA, Good SV, Yegorov S, Bogerd J, Gooley PR. (2018). Pharmacology & Therapeutics
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Samuel CS, Royce SG, Hewitson TD, Denton KM, Cooney TE, Bennett RG. (2017). Br J Pharmacol
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Valkovic AL, Bathgate RAD, Samuel CS, Kocan M. (2019). Mol Cell Endocrinol
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Chen TY, Li X, Hung CH, Bahudhanapati H, Tan J, Kass DJ, Zhang Y. (2020). Mol Genet Genomic Med
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Samuel CS, Li Y, Wang Y, Widdop RE. (2024). Br J Pharmacol
Primary research
7Alam F, Gaspari TA, Kemp-Harper BK, Low E, Aw A, Ferens D, Spizzo I, Jefferis AM, Praveen P, Widdop RE, et al. (2023). Biomedicine & Pharmacotherapy
- 8
Praveen P, Mauro AG, van Heerden A, Alim MS, Bathgate RAD, Widdop RE, Kocan M, Hossain MA. (2023). Int J Mol Sci
- 9
Bhuiyan S, Shen M, Chelvaretnam S, Tan AY, Ho G, Hossain MA, Widdop RE, Samuel CS. (2021). FASEB Journal
- 10
Devarakonda T, Mauro AG, Guzman G, Hovsepian S, Cain C, Das A, Praveen P, Hossain MA, Salloum FN. (2020). Journal of the American Heart Association
- 11
Welch NG, Mukherjee S, Hossain MA, Praveen P, Werkmeister JA, Wade JD, Bathgate RAD, Winkler DA, Thissen H. (2019). ACS Applied Materials & Interfaces
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Hossain MA, Kocan M, Yao ST, Royce SG, Nair VB, Siwek C, Patil NA, Harrison IP, Rosengren KJ, Selemidis S, et al. (2016). Chemical Science
- 13
Marshall SA, O'Sullivan K, Ng HH, Bathgate RAD, Parry LJ, Hossain MA, Leo CH. (2017). Eur J Pharmacol
- 14
Devarakonda T, Mauro AG, Cain C, Das A, Salloum FN. (2021). JACC Basic Transl Sci
- 15
D'Ercole A, Nistri S, Pacini L, Carotenuto A, Santoro F, Papini AM, Bathgate RAD, Bani D, Rovero P. (2022). Front Pharmacol
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Erlandson SC, Rawson S, Osei-Owusu J, Brock KP, Liu X, Paulo JA, Mintseris J, Gygi SP, Marks DS, Cong X, Kruse AC. (2023). Nat Chem Biol
- 17
Handley TNG, Praveen P, Tailhades J, Wu H, Bathgate RAD, Hossain MA. (2023). Int J Mol Sci
- 18
Granberg KL, Sakamaki S, Fuchigami R, Niwa Y, Fujio M, Kato H, et al. (2024). J Med Chem
- 19
Poirier B, Pasquier O, Chenede X, Corbier A, Prigent P, et al. (2024). Br J Pharmacol
- 20
Erlandson SC, Wang J, Jiang H, Osei-Owusu J, Rockman HA, Kruse AC. (2024). Mol Pharm
- 21
Somanader-Livera DV, Wei C, Wang C, Li Y, Ferens D, Salimova E, Selomulya C, Hossain MA, Samuel CS, Chakraborty A. (2025). J Biomed Sci
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Agoulnik IU, Kaftanovskaya EM, Myhr C, Bathgate RAD, Kocan M, Peng Y, Lindsay RM, DiStefano PS, Agoulnik AI. (2024). Biochem Pharmacol