Kisspeptin-10

A synthetic C-terminally amidated decapeptide corresponding to the C-terminal fragment of the KISS1 gene product, characterized in cryo-EM structural studies of class A GPCR complexes.

Molecular Profile

Type
Linear decapeptide (C-terminally amidated), RF-amide peptide family
Molecular formula
C63H83N17O14
Molecular weight
1302.4 g/mol
CAS number
374675-21-5
Amino acids
10
Sequence
Tyr-Asn-Trp-Asn-Ser-Phe-Gly-Leu-Arg-Phe-NH2
Modification
C-terminal amidation (RF-amide motif)

Mechanism & Target Class

Kisspeptin-10 is a linear decapeptide corresponding to the conserved C-terminal segment shared by the four naturally occurring kisspeptins (KP-54, KP-14, KP-13, KP-10), all of which terminate in an arginine-phenylalanine-amide (RF-amide) motif. C-terminal amidation is the single most critical structural determinant of the molecule's binding conformation, and structure-activity studies establish that the terminal amide is required for high-affinity engagement. Cryo-EM studies of KP-10 bound to its class A G-protein-coupled receptor complex define a three-segment binding mode in which the N-terminal residues (Tyr-Asn-Trp-Asn-Ser-Phe) sit between the receptor extracellular loops and the C-terminal RF-amide inserts into the transmembrane core (PDB 8ZJD), with Phe6, Arg9, and Phe10 identified as residues central to the interaction. KP-10 belongs to the RF-amide peptide family, alongside neuropeptide FF, prolactin-releasing peptide, and QRFP.

Storage & Handling

Lyophilized
Store lyophilized powder sealed and protected from light, heat, and moisture; long-term storage at -20°C is standard for peptide research materials.
Handling
As a small linear peptide, KP-10 is subject to enzymatic degradation in biological matrices; its in vivo plasma half-life is notably shorter than that of kisspeptin-54. Aliquoting is recommended to preserve integrity.

Primary Database

PubChem CID 25240297 ↗

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